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ERX2525150: PacBio RS sequencing
1 PACBIO_SMRT (PacBio RS) run: 163,482 spots, 3.7G bases, 12.1Gb downloads

Submitted by: Centro Biologia Molecular Severo Ochoa (CBMSO)
Study: De novo assembly of the L. braziliensis MHOM/BR/75/M2904 strain using sequence reads generated by two different NGS platforms, Illumina and PacBio. As a result, we are providing a complete assembly of the genome consisting of 35 contigs, one for each chromosome. In addition, we are providing a complete annotation of predicted genes.
show Abstracthide Abstract
Leishmania braziliensis is a parasitic protist, the causative agent of a type of leishmaniasis known as mucocutaneous leishmaniasis (MCL), in which lesions erupt at many sites on the nasopharyngeal mucosa as consequence of parasite multiplication. A genome draft for the MHOM/BR/75/M2904 strain of L. braziliensis was published in 2007 (Peacock et al. 2007. Nat Genet 39: 839). However, the assembly was not complete, and the chromosomes were rather scaffolds consisting of juxtaposed genomic fragments. In this project, we have carried out a de novo assembly of the genome for this L. braziliensis strain using sequence reads generated by two different NGS platforms, Illumina and PacBio. As a result, we are providing a complete assembly of the genome consisting of 35 contigs, one for each chromosome. In addition, we are providing a complete annotation of predicted genes.
Sample: L. braziliensis MHOM/BR/75/M2904 strain for genome assembly. PacBio raw reads.
SAMEA1068703 • ERS2373969 • All experiments • All runs
Library:
Name: unspecified
Instrument: PacBio RS
Strategy: WGS
Source: GENOMIC
Selection: unspecified
Layout: SINGLE
Runs: 1 run, 163,482 spots, 3.7G bases, 12.1Gb
Run# of Spots# of BasesSizePublished
ERR2505823163,4823.7G12.1Gb2018-12-02

ID:
6857830

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